ESPN 51th Annual Meeting

ESPN 2018


 
TUBULOINTERSTITIAL EXPRESSION OF KIDNEY INJURY MOLECULE-1 IN CHILDREN WITH CHRONIC GLOMERULOPATHIES
GORDANA MILOŠEVSKI-LOMIĆ 1 SANJA RADOJEVIĆ-ŠKODRIĆ 3 GORDANA BASTA-JOVANOVIĆ 3 MIRJANA KOSTIĆ 2 BILJANA MILIČIĆ 4 DUŠAN PARIPOVIĆ 2 SRĐAN NIKOLOVSKI 1 BRANKICA SPASOJEVIĆ-DIMITRIJEVA 2 IVANA GOJOVIĆ 1 MIRJANA CVETKOVIĆ 1 DIVNA KRUŠČIĆ 1 AMIRA PECO-ANTIĆ 2

1- UNIVERSITY CHILDREN’S HOSPITAL, NEPHROLOGY DEPARTMENT, BELGRADE, SERBIA
2- UNIVERSITY CHILDREN’S HOSPITAL, NEPHROLOGY DEPARTMENT, BELGRADE, SERBIA; SCHOOL OF MEDICINE, UNIVERSITY OF BELGRADE, BELGRADE, SERBIA
3- INSTITUTE OF PATHOLOGY, SCHOOL OF MEDICINE, UNIVERSITY OF BELGRADE, SERBIA; SCHOOL OF MEDICINE, UNIVERSITY OF BELGRADE, BELGRADE, SERBIA
4- DEPARTMENT FOR MEDICAL STATISTICS AND INFORMATICS, SCHOOL OF DENTAL MEDICINE UNIVERSITY OF BELGRADE, BELGRADE, SERBIA
 
Introduction:

Early identification of the markers of tubulointerstitial (TIN) lesions and prompt treatment of TIN may give chance for healing or slowing the progression of glomerular disease. Retrospective study was designed to examine the prognostic value of tissue kidney injury molecule-1 (KIM-1) expression retrospectively in biopsies sampled in 38 pediatric patients aged 9.05±5.20 years at the time of the clinical onset of chronic glomerular disease (CGD).

Material and methods:

Tissue KIM-1 expression was determined immunohistochemically, and KIM-1 staining was scored semiquantitatively as well as TIN inflammation, atrophy, and fibrosis. Kidney function-KF (Schwartz’s formula), blood pressure and proteinuria/spot urine were evaluated at the time of biopsy and at the end of follow up.

Results:

Patients with moderate to diffuse KIM-1 staining had more pronounced mild tubular atrophy and interstitial fibrosis (p<0.05) as well as a greater expression of interstitial alpha-smooth muscle actin antibody-αSMA (p<0.01) compared with patients who had negative or focal KIM-1 TIN staining. Significantly positive correlation was found between KIM-1 and αSMA expression (p<0.05). In patients with decreased KF compared with patients with normal KF a moderate degree of KIM-1 expression (p<0.05); moderate and diffuse α SMA expressions (p<0.01); a mild and moderate tubulointerstitial inflammation (p<0.01) as well as moderate and severe tubular atrophy and mild fibrosis (p<0.01) were more prevalent. Expression of α SMA was the best predictor of KIM-1 kidney tissue expression (p<0.05).

Conclusions:

We conclude that KIM-1 in kidney biopsy tissue might serve as an additional sensitive biomarker of re-differentiation of tubular epithelial cells in children with CGD.