ESPN 51th Annual Meeting

ESPN 2018


 
USING 1,25(OH)2 VITAMIN D3 AND PARICALCITOL IN RATS, IN WHICH THE NEPHROTIC SYNDROME IS INDUCED BY PUROMYCIN (PAN), IT WAS EFFECT OF THE LEVEL OF PROTEINURIA, PODOCIN AND NEPHRIN MRNA WITH PCR
HAMDI METIN 1 PELIN ERTAN 1 AHMET KESKINOĞLU 2 ELGIN TÜRKÖZ ULUER 3 SIRRI ÇAM 4 PEMBE KESKINOĞLU 5 MUHAMMET BURAK BATIR 4 DAMLA AKOĞULLARI 3

1- MANISA, CELAL BAYAR UNIVERSITY, DEPARTMENT PEDIATRIC NEPHROLOGY/MANISA/TURKEY
2- EGE UNIVERSITY, DEPARTMENT PEDIATRIC NEPHROLOGY/İZMIR/TURKEY
3- MANISA, CELAL BAYAR UNIVERSITY, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY/MANISA/TURKEY
4- MANISA, CELAL BAYAR UNIVERSITY, DEPARTMENT OF MEDICAL GENETIC/MANISA/TURKEY
5- DOKUZ EYLUL UNIVERSITY, DEPARTMENT OF BIOSTATISTICS/İZMIR/TURKEY
 
Introduction:

Nephrotic syndrome is a disease that may give rise to the serious morbidities after childhood period. Antiproteinuric and renoprotective effects are tried in several medicines.We aimed to investigate the effect of 1,25 (OH)2D3 vitamin and paricalcitol proteinuria, renal tissue histology, podocin and nephrin upon mRNA.

Material and methods:

28 number of rats has been divided into 4 groups. Experimental nephrotic syndrome was provided with 10mg/100gr of puromycin. Group 1 has been determined as a healthy control group, Group 2 as a nephrotic syndrome group formed by PAN, Group 3 as a vitamin D group, Group 4 as a paricalcitol group. No medication has been realized to Group 1. Only puromycin application was performed to Group 2. 1,25 (OH)2 D3 application has been realized intraperitoneally to Group 3 in dose of 0,4 µg/kg/day every day for 7 days  after performing the application of puromycin. Paricalcitol was applied intraperitoneally to Group 4 in dose of 240ng/kg/day every day for 7 days after performing the use of puromycin. Spot urine samples were collected after performing massaged on the 0th, 4th and 7th days. After taking renal tissue from the rats for 7 days, rats were sacrificed by cervical dislocation method.

Results:

An increase of urine protein/creatinine ratio in nephrotic syndrome has been found significant (p=0.015). Besides, increase of protein/creatinine within days is significant also in groups of paricalcitol and vitamin D (p=0,008,p:0,030). However, when compared among groups, the presence of a statistically-significant difference between urine protein/creatinine ratio on 0th day makes the comparison between the groups meaningless in the following days (p=0,028). No significant difference could be detected between the groups in mRNA expression of nephrin (p=0,721). In mRNA expression of podocin, no statistically significant increase has been detected according to  nephrotic syndrome group (p = 0.003). Glomerular hypersolarity was determined in the nephrotic syndrome group in performed histological studies. Minimal glomerular hypersolarity was detected in paricalcitol and vitamin D group. No significant immunoreactivity could have been detected in the immunohistochemical studies realized for podocin and nephrin (p= 0,356, p= 0,475).

Conclusions:

As a result, paricalcitol and Vitamin D, we are aware that there are antiproteinuric and renoprotective effects. Although we have observed numerically this effect in the present study, statistical significance has been achieved.