ESPN 51th Annual Meeting

ESPN 2018


 
Salivary urea and creatinine in children with renal dysfunction
PODRACKA LUDMILA 1 CELEC PETER 2 TóTHOVá ĽUBOMíRA 2 ŠEBEKOVá KATARíNA 2

1- DEPT. PEDIAT. UNIV.CHILDRENS HOSPITAL AND FACULTY MEDICINE
2- INSTITUTE OF MOLECULAR BIOLOGY, FACULTY MEDICINE, COMMENIUS UNIVERSITY, Bratislava, Slovakia
 
Introduction:

 Plasma creatinine and urea are commonly used markers of kidney function in both, acute and chronic renal failure. The needed repeated blood collection is associated with pain, stress and might lead to infections. Saliva has the potential to be a non-invasive alternative diagnostic fluid. The use of saliva in clinical practice is limited, since many factors affect the concentration of salivary biomarkers.

Material and methods:

 The aim of our study was to analyze salivary creatinine and urea in children with acute and chronic renal failure. Saliva samples were studied from 39 children with renal dysfunction (13 Tx, 13Dx, 3 AKI, 10 with CKD 2.-4. KDIGO) and 10 healthy children. Un-stimulated whole saliva was collected between 9 to 11 am from all individuals. The saliva was collected using spitting method in disposable, sterile, plastic containers (Nalgene Sterile Sample Bottles) with measurement markings. The collected samples were submitted for analysis of urea and nitrogen by an Automated Bio analyser. 

Results:

 The mean salivary creatine  in dialyzed children was significantly higher compared to transplanted patients (x= 75,35 +/-53 umol/l resp. x=31,48+/- 16,79 p<0.01).  The salivary urea did not differ significantly between children with various degree of renal dysfunction. However, the salivary urea was higher in comparison to the controls (Tx x= 1.58±1.78mmol/l; Dx x=1,164+/-1,401mmol/l, CKD x=1,948+/-2,381mmol/l ns. controls x=0,36+/-0,09832mmol/l, respectively).

Conclusions:

 Our preliminary results indicate that saliva can be used as a noninvasive alternative to serum for creatinine estimation. Further studies are needed to monitor the dynamics of salivary markers of renal function and to reveal determinants of their variability.