ESPN 51th Annual Meeting

ESPN 2018


 
Do the gender and age of children effect the in vitro growth rate of uropathogenic Escherichia coli in urine?
ALPER SOYLU 1 MERAL KARAMAN 2 MERAL TORUN BAYRAM 1 BATUHAN KÜÇÜKALI 1 SEVCAN İMAM 1 SALIH KAVUKÇU 1

1- DOKUZ EYLÜL UNIVERSITY MEDICAL FACULTY DEPARTMENT OF PEDIATRICS
2- DOKUZ EYLÜL UNIVERSITY MEDICAL FACULTY DEPARTMENT OF MICROBIOLOGY
 
Introduction:

UTI is more frequent in infants and girls. Uropathogenic E.coli (UPEC) is the most frequent organism. Urine composition, associated with urolithiasis, changes with race, sex and age in healthy people. It was shown that urine of trauma patients facilitated E.coli growth. We hypothesized that UTI rate in children might be affected by urine compositional changes by age and gender.

Material and methods:

Children presenting due to non-UTI causes were enrolled. Sterile bag/clean catch urine samples were processed as follows: 0.1 mL UPEC suspension (2x103 cfu/mL) was added to 0.9 mL urine, incubated for 4 hours, and 0.1 mL of this last suspension (2x102 cfu/mL) was inoculated to blood and EMB agars, incubated for 24 hours and colony counts were assessed. Children of both genders and of various age groups were compared for the number of urine samples with any UPEC growth and for colony counts.

Results:

84 urine samples were included (32 girls). UPEC growth was not affected by gender in general. However, in children >5 years, it was higher in girls than in boys (16/18 vs 20/34, p=0.025; OR 5.6). UPEC growth in infants <2 years was higher than in children >2 years (16/17 vs 48/67, p=0.052, OR 6.3). Colony counts were not different between genders and various age groups. However, in 4 infants (3 male) colony count reached >105 col/mL; three of them were 2-month of age and one was 4-month of age. 

Conclusions:

 In vitro UPEC growth is facilitated in urine samples of young children and in girls >5-year of age. This finding suggests that urine compositional changes by gender and age might contribute to bacterial growth in urine.