ESPN 51th Annual Meeting

ESPN 2018


 
Conventional Growing Piglets As Potential Juvenile Animal Model for Preclinical Pharmaceutical Research: :Postnatal Maturation of the Glomerular Filtration Rate
Elke Gasthuys 1 Joske Millecam 1 Mathias Devreeze 1 Joris Delanghe 2 Johan Vande Walle 2 M Heynderickx 3 Siska Croubels 1

1- Safepedug,Veterinary School, Ugent
2- Safepedrug, Ugent,GEO2
3- Faculty of Sciences, Ghent
 
Introduction:

 Adequate animal models are required to study the preclinical pharmacokinetics (PK), pharmacodynamics (PD) and safety of drugs in the pediatric subpopulation. Over the years, pigs were presented as a potential animal model, since they display a high degree of anatomical and physiological similarities with humans.

Material and methods:

  The kidneys play a pivotal role in the PK and PD of various drugs, therefore, the glomerular filtration rate (GFR) measured as clearance of endogenous creatinine (Jaffe and enzymatic assay) and exo-iohexol was determined in conventional piglets aging 8 days (n = 16), 4 weeks (n = 8) and 7 weeks (n = 16). The GFR data were normalized to bodyweight (BW), body surface area (BSA) and kidney weight (KW). Body Surface area is measured by X-ray Tomography

Results:

GFR mesaured with iohexol and creatinine were comparible. Normalization to BW showed a less pronounced increase from 3.55 to 4.31 mL/min/kg. Moreover, to facilitate the assessment of the GFR in growing piglets in subsequent studies, a formula was elaborated to estimate the GFR based on plasma creatinine and BW, namely eGFR =1.879 × BW1.092

Conclusions:

 The postnatal development of the GFR was comparable with humans, rendering the piglet a convenient juvenile animal model for studying the PK, PD and safety of drugs in the pediatric subpopulation.