ESPN 51th Annual Meeting

ESPN 2018

Conventional Growing Piglets As Potential Juvenile Animal Model for Preclinical Pharmaceutical Research: :Postnatal Maturation of the Glomerular Filtration Rate
Elke Gasthuys 1 Joske Millecam 1 Mathias Devreeze 1 Joris Delanghe 2 Johan Vande Walle 2 M Heynderickx 3 Siska Croubels 1

1- Safepedug,Veterinary School, Ugent
2- Safepedrug, Ugent,GEO2
3- Faculty of Sciences, Ghent

 Adequate animal models are required to study the preclinical pharmacokinetics (PK), pharmacodynamics (PD) and safety of drugs in the pediatric subpopulation. Over the years, pigs were presented as a potential animal model, since they display a high degree of anatomical and physiological similarities with humans.

Material and methods:

  The kidneys play a pivotal role in the PK and PD of various drugs, therefore, the glomerular filtration rate (GFR) measured as clearance of endogenous creatinine (Jaffe and enzymatic assay) and exo-iohexol was determined in conventional piglets aging 8 days (n = 16), 4 weeks (n = 8) and 7 weeks (n = 16). The GFR data were normalized to bodyweight (BW), body surface area (BSA) and kidney weight (KW). Body Surface area is measured by X-ray Tomography


GFR mesaured with iohexol and creatinine were comparible. Normalization to BW showed a less pronounced increase from 3.55 to 4.31 mL/min/kg. Moreover, to facilitate the assessment of the GFR in growing piglets in subsequent studies, a formula was elaborated to estimate the GFR based on plasma creatinine and BW, namely eGFR =1.879 × BW1.092


 The postnatal development of the GFR was comparable with humans, rendering the piglet a convenient juvenile animal model for studying the PK, PD and safety of drugs in the pediatric subpopulation.