ESPN 51th Annual Meeting

ESPN 2018


 
The utility of urinary CD80 as a diagnostic maker in patients with renal diseases
Shogo Minamikawa 1 Kandai Nozu 1 Shinya Ishiko 1 Yuya Aoto 1 Nana Sakakibara 1 China Nagano 1 Jyunya Fujimura 1 Tomohiko Yamamura 1 Yuko Shima 2 Koichi Nakanishi 3 Kazumoto Iijima 1

1- Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
2- Department of Pediatrics, Wakayama Medical University, Wakayama, Japan
3- Department of Child Health and Welfare, University of the Ryukyus, Naha, Japan
 
Introduction:

CD80 is expressed on antigen-presenting cells and regulates the activation of T-cells. It is also reported to be expressed on podocytes when they are cultured with serum from minimal change disease (MCD) patients. In the past, it had been suggested that both urinary CD80 level and expression of CD80 on kidney tissue can be used as differential diagnostic markers between MCD and other renal diseases including focal segmental glomerular sclerosis (FSGS). But some previous reports showed the negative data for the usefulness of CD80 as a diagnostic marker. Therefore, we examined the urinary CD80 levels for patients with various kidney diseases, and evaluated the usefulness of urinary CD80 as a diagnostic biomarker.

Material and methods:

We collected 160 urine samples from 55 patients with MCD (n=31), FSGS (n=4), inherited nephrotic syndrome (n=4), Alport syndrome (n=5), other glomerular diseases (n=11), and 30 healthy controls. And we measured the levels of urine CD80 using sandwich ELISA assay.

Results:

The urine CD80 (ng/gCr) (mean±SE) levels were significantly higher in MCD in relapse (297.3±84.5), FSGS (450.3±278.6), or inherited nephrotic syndrome (382.8±227.9) than MCD in remission (56.2±9.9) (p<0.05). There was no significant difference of the urinary CD80 levels between MCD in remission and controls (65.3±3.6). Surprisingly, the elevation of urinary CD80 was observed even in patients with genetically proven inherited NS which is not related to T cell activation. Additionally, urinary CD80 had a positive correlation with urinary protein levels.

Conclusions:

In contrast to the previous reports, our results showed that urinary CD80 was not reliable differential diagnostic marker between MCD in relapse and FSGS or other kidney diseases including inherited kidney diseases. The level of urinary CD80 was increased in all patients with active kidney diseases.