ESPN 51th Annual Meeting

ESPN 2018


 
CORRELATION OF THOMA CELL COUNTING CHAMBER WITH AUTHOMATED BLOOD COUNTER AND URINE ANALYSER IN DIAGNOSIS OF PERITONITIS
ERKIN SERDAROGLU 1 AYSEN TOKTAY 1 SÜKRAN KESKIN GOZMEN 1 SERKAN KEMER 1 CEMALIYE BASARAN 1

1- UNIVERSITY OF HEALTH SCIENCES DR. BEHCET UZ CHILDREN’S HOSPITAL DEPARTMENT OF PEDIATRIC NEPHROLOGY
 
Introduction:

Peritonitis remains the most important complication in peritoneal dialysis (PD). Abdominal pain, nause and vomiting with cloudy peritoneal effluent and peritoneal fluid pleocytosis (white blood cell >100/mm3, >50% polimorphs) are the main findings of peritonitis. Cell counting on Thoma cell counting chamber (TCCC) with optical microscope is gold standard for fluid pleocytosis. In this study we aimed to correlate TCCC with automated blood counter and urine analysis device results in peritoneal fluid examination.

Material and methods:

167 samples of 23 pediatric patients who underwent PD were evaluated microscopically in TCCC and authomated blood counter (Mindray BC-6800) and urine analysis device (Labomat Urised2).

Results:

Mean number of cells detected on TCCC, authomated blood counter and urine analyser were 115±238/mm3 (median = 10), 306±829/mm3 (median = 50) and 27±79/mm3 (median = 3) respectively. TCCC and automated blood counter correlated well (r=0.74) and had a high agreement with AUC 0.93 in ROC curve. In dipstick method; “0”, “1+”, “2+”, “3+” result percentages were determined as 61%, 14%, 15% and 10% respectively. When compared with standard TCCC method, the negative predictive value of the dipstick test was 96%, sensitivity and specifity were 91 and 78 for value of greater than 100/mm3 cell (AUC=0.84).

Conclusions:

These results suggest that automated cell counter can be used instead of TCCC as an easier and faster method. Also, urine dipstick may be useful to detect peritonitis as an home-based method.